A passenger train serving the Thessaloniki-Larisa route derailed as it arrived at Platy station in Imathia, Central Macedonia, early Thursday morning.
The accident caused damages to the railway carriages of train 1591 but there were no injuries, operator TRAINOSE said in a statement.
The company said it is investigating the circumstances of the incident.
An engine failure on a train serving the Athens suburban railway (Proastiakos) route on Monday was expected to result in cancellations and delays.
The problem occurred at around 9 a.m. on a train en route to Athens International Airport from Piraeus, leading to the train being stopped at Koropi.
Trainose said that engineers were working on restoring services.
State transport companies recorded a decline in revenues and an increase in losses last year, according to official figures.
Revenues fell 1.8 percent from 2016 to 289.3 million euros, with the 84-million-euro increase in state subsidies failing to plug the gap.
For every euro the transport companies earned as operating revenues last year, they got another from taxpayers.
One woman died and two people were injured near Tithorea in central Greece on Thursday morning, when the car she was driving collided with a speeding train.
Rail company TRAINOSE said train 1511 was heading from Lianokladi to Athens when it collided with a vehicle that ignored the bars on the level crossing.
The high-speed "Silver Arrow" train that will connect Thessaloniki to Athens in just 3.5 hours, made a test run on Saturday from the northern port city to the town of Katerini.
According to Transport Minister Nikos Mavraganis, the electrification of the final 52-kilometer stretch of the Athens-Thessaloniki rail corridor will take place by end of the year.
Italian-owned Greek rail operator Trainose is expected to unveil a high-speed train that will reduce the journey between Athens and Thessaloniki in northern Greece to 3 hours and 20 minutes.
Nicknamed "Silver Arrow," the Frecciargento ETR 485 will be presented at next month's Thessaloniki International Fair.
Greece's second private railway company, following the privatization of Trainose, is about to make its first journey on the Greek public rail network. Greek-Austrian consortium Rail Cargo Logistic Goldair is active only in cargo transport, as the name suggests, and its first outing using its own carriages will serve the Sindos-Idomeni route in northern Greece.